Antibacterial activity of Algerian Punica granatum Linn. extracts (Juice, pericarp and seed) against clinical isolates of β-lactamase producing methicillin resistant Staphylococcus aureus and extended-spectrum beta-lactamase ESBL-producing



1Bioconversion, Microbiological engineering and Health security, SNV Faculty, University of Mascara - 29000 Algeria

2 BPC Department, SNV Faculty, Blida 1 University. Algeria.

3Department of Pharmacognosy, College of Pharmacy, King Saud University P.O. Box 2457, Riyadh 11451, Saudi Arabia


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Punica granatum is ethnomedicinal important plant and depicted ameliorating medicinal value which used for the treatment of various diseases. It is an important medicinal plant in Algeria. The present study deals with the evaluation of the in vitro antimicrobial potential of four different extracts of Algerian Punica granatum Linn by using agar diffusion methods and minimum inhibitory concentration (MIC)-determination. Moreover, the extracts were investigated for their polyphenolic content. The quantitative assays of total polyphenols by the Folin-Ciocalteu test revealed the richness of the different pomegranate extract by polyphenol, content varies from 18.69 to 116.1mg GAE/100g of fruit this value. The phenolic compounds were abundant in acetone, methanolic and aqueous extracts. It’s depends on the polarity of the solvent and the extraction method. According to the results of the aromatogramme, all the phenolic extracts showed antibacterial effect against all extended-spectrum beta-lactamase ESBL-producing
Enterobacteriaceae with a strong inhibitor with MIC <500
μg/ml power. This effect is especially marked by the pericarp extract against Escherichia coli, Citrobacter freundii and Klebseilla pneumoniae . On the other hand the extract Punica granatum (pericarp and juice) showed a broad-spectrum of antibacterial activity with an inhibition zone size of 11 mm to 29 mm, against β-lactamase producing methicillin resistant Staphylococcus aureus strains.



Keywords: Punica granatum,, Enterobacteriaceae producing β-lactamase, polyphenols, Antimicrobial activity, Staphylococcus aureus, MRSA, extended-spectrum beta-lactamase ESBL-producing