Synergetic hepatoprotective effect of the phenolic fractions obtained from Ficus carica dried fruit and extra virgin olive oil on CCl4–Induced Oxidative Stress and Hepatotoxicity in Rats




1Bioconversion, Microbiological engineering and Health security, SNV Faculty, Université of Mascara - 29000 Algeria

2 BPC Department, SNV Faculty, Blida 1 University. Algeria.

3Grupo de Investigacio´n en Polifenoles, Unidad de Nutricio´n y Bromatologı´a, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain

4Department of Pharmacognosy, College of Pharmacy, King Saud University P.O. Box 2457, Riyadh 11451, Saudi Arabia

5Physiologie de la nutrition et sécurité alimentaire, University of El Sania, Oran Algeria   

6 Laboratoire d’ecologie animale E.N.S de Kouba, Alger, Algerie


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The present study aimed to investigate the possible hepatoprotective effect of the dried fruits of Ficus caraca L., extra virgin olive oil, their phenolic fractions, and their synergistic interactions on enhancing hepatoprotective properties in on CCl4–Induced Oxidative Stress and Hepatotoxicity in Rats. The hepatoprotective activity was evaluated by measuring levels of serum marker enzymes like serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and alkaline phosphatase (ALP). The serum levels of bilirubin were also estimated. The histological studies were also carried out to support the above parameters. Administration of extra virgin olive oil (EVOO) (3.0 g/kg/day/p.o) and their methanol extract (MEEVOO) (2,1mg/kg/day/i.v) 7 days significantly (p<0.05) prevented CCl4-induced elevation of levels of serum GPT, GOT, and ALP. The methanol dried fig extract (38,07 mg/kg/day/i.v) was found hepatoprotective more potent than the dried figs (4g/kg/day/p.o). The serum GTP and GOT levels were increased due to hepatic damage induced by CCl4 and were found to be decreased in dried fig and their aqueous extract treated groups. Administration of methanol extra virgin olive extract (MEEVOO) with the methanol dried fig extract simultaneously, significantly (p< 0.05) prevented CCl4-induced elevation of levels of serum GPT, GOT, and ALP. These data suggest the synergistic functionality of these phytochemicals in dried figs and extra virgin olive oil. Treatment of rats with CCl4 led to a marked increase in lipid peroxidation as measured by malondialdehyde (MDA). Hepatic antioxidant enzyme, Catalase (CAT) was significantly decreased in all treated animals. A comparative histopathological study of liver exhibited almost normal architecture, as compared to CCl4 treated control group. The observed hepatoprotective effect is believed to occur due to antioxidant properties of the contents of extra virgin olive oil and dried figs extract, which may provide a new drug to be used for prophylaxis against liver diseases and it validates its folkloric use by Algerian people.


Keywords: Extra virgin olive oil, dried figs, antioxidant, carbon tetrachloride, Ficus carica, hepatoprotection, lipid peroxidation